Description | CD4isasingle-passtypeImembraneproteinexpressedonthesurfaceofThelper/inducercells,regulatoryTcells,monocytes,macrophages,dendriticcellsandmicroglia. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | CD4transfectedmouseT-cellhybridoma3DTfollowedbyCD4+humanT-cellCEMcellline |
Clone | CA-4 |
OtherNames | T-cellsurfaceglycoproteinCD4;T-cellsurfaceantigenT4/Leu-3;W3/25antigen;CD4mut; |
Accession | P01730CD4_HUMAN;P05540CD4_RAT; |
Producedin | Mouse |
Purity | IgG |
Applications | Immunohistochemistry(IHC-Frozen).Aconcentrationof1.0µg/mlisrecommendedtodetectCD4informalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Thespecificityofthisantibodyhasbeenconfirmedbyimmunohistochemistryagainsttheantigen. |
Cross-reactivity | Human;rat; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpracticeisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Liquid(0.5ml).50%glycerol,0.9mgNaCland0.2mgNa2HPO4 |
Reconstitution | TheliquidformulationshouldbedilutedinPBS(pH7.4) |
Storage | Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
References | 1.AlmoldaB.etal.CD4microglialexpressioncorrelateswithspontaneousclinicalimprovementintheacuteLewisratEAEmodel.JNeuroimmunol.2009Apr30;209(1-2):65-80. 2.JordanC.A.etal.Infectionofbrainmicroglialcellsbyhumanimmunodeficiencyvirustype1isCD4dependent.JVirol.1991Feb;65(2):736-42. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。