Description | Calbindin-Disahighlyconserved28kDaproteinthatbelongstoafamilyoflowmolecularweightcalcium-bindingproteins(CaBPs).Calbindin-Dhas4activecalciumbindingdomains.Itplaysaroleintheregulationofcalciumandcalciumrelatedsignallinginneuronsandduringembryodevelopment.Thisantibodybindsonlytocalbindin-D-28Kandnotwithotherfamilymemberssuchascalretininandcalbindin-D-9K. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | BovinekidneyCalbindin-D |
Clone | CB-D7 |
OtherNames | CALB1_BOVIN;CALB1; |
Accession | P04467CALB1_BOVIN |
Producedin | Mouse |
Purity | IgG1 |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.BovineCalbindin-Dhasapredictedlengthof261residuesandaMWof28kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetectCalbindin-Dinformalinfixedandparaffinembeddedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
SpeciesAgainst | Theantibodyworksonratandisexpectedtoworkonhuman,mouseandbovine. |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。