Description | Actinisoneofthemostabundantandhighlyconservedproteinsofeukaryotes.Mammalianactinsaretheproductofsixdifferentgeneswithdifferingdistributionpatternsincelltypesandintissues.Themolecularweightofallsixproteinsis42kDa,andoneormoreactinsisfoundinessentiallyeverytypeofcrudecellularandtissueextract.Asaresultantibodiestoactinarewidelyusedasinwesternblottingstandards.Thesecanbeusedtoverifythatthevariousstepsofthewesternblottingprocedurehavebeenperformedcorrectly.Inaddition,actinisregardedasa"housekeeping"proteinwhichisgenerallynotalteredmuchinexpressionasaresultofexperimentalmanipulations.Soquantitationoftheactinbandonthewesternisusedasastandardagainstwiththebanddensityofotherproteinscanbecompared.Themonoclonalbindsallsixactinisotypes(ACTA1,ACTA2,ACTC1,ACTB,ACTG1andACTG2)verystronglyonwesternblots.Itisaveryeffectiveblottingstandardwhichcanworkonanycelltypeortissueextract.Italsoworksinimmunocytochemicalexperiments,bindingstronglyandcleanlytofilopodia,membranerufflesandstressfibers,allknowntoberichinactin. |
Antigen | Actinpreparedfrombovinebrain. |
AntibodyType | Monoclonal |
Isotype | IgG1 |
Producedin | Mouse |
Applications | WesternBlotting(WB),Immunocytochemistry(IC)andFlowcytometry.Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Use2ug/10^6cellsforFlowcytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Theantibodyreactswitha42kDabandbyWesternblotonacrudeextractfromHeLacells.Ithasalsobeenusedsuccessfullyforimmunocytochemistry. |
SpeciesAgainst | Human,rat,mouse,porcineandbovine.Itisexpectedthatitwillworkonothermammaltissues. |
AntibodyAgainst | Actin |
Form | LyophilizedfromPBS.Contains5%trehalose. |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilizedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
GeneralReferences | 1.Vandekerckhove,J.andWeber,K.Atleastsixdifferentactinsareexpressedinahighermammal:ananalysisbasedontheaminoacid.sequenceoftheamino-terminaltrypticpeptide.J.Mol.Biol.126:783802(1978). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。