Description | Neurofilamentscontainthreeintermediatefilamentproteins:light(68kDa),medium(160kDa)andheavy(200kDa).Neurofilamentheavy(NF200orNF-H)isphosphorylatedanditisthoughtthatthisresultsintheformationofinterfilamentcrossbridgesthatareimportantinthemaintenanceofaxonalcaliber.ThisantibodybindsprimarilytothephosphorylatedaxonalformsofNF-H. |
BatchNo. | Seeproductlabel |
Unitsize | 50µl |
Antigen | PurifiedbovineNeurofilamentHeavy(NF-H) |
Isotype | IgY |
OtherNames | NF-200;NF200;NF-H;NEFH;N52;Neurofilamentheavypolypeptide;NeurofilamenttripletHprotein;200kDaneurofilamentprotein;KIAA0845;NFH; |
Accession | P12036NFH_HUMAN; |
Producedin | Chicken |
Applications | WesternBlotting(WB),Immunocytochemistry(IC)andELISA.SuggesteddilutionforWBis1:100,000-1,000,000.SuggesteddilutionforICis1:50,000-100,000.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThisantibodyreactswithphosphorylatedNF-Handisseenasabandofapprox200kDainWB.RefertopublicationbyShawetal(2005)fortheuseofthisantibodyinanELISAtodetectNF-H. |
AntibodyAgainst | NeurofilamentHeavyphosphorylated |
Cross-reactivity | Hu,Rat,Ms,Fel,Chk.Predictedtoreactwithothermammaliantissuesduetosequencehomology. |
BlastURL | Clickhere |
Form | Lyophilisedwith5%trehalose |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.JarjourA.A.etal(2007)Maintenanceofaxo-oligodendroglialparanodaljunctionsrequiresDCCandnetrin-1.JNeurosci.2008Oct22;28(43):11003-14. 2.PearseD.D.etal(2007)TransplantationofSchwanncellsand/orolfactoryensheathinggliaintothecontusedspinalcord:Survival,migration,axonassociation,andfunctionalrecovery.Glia.2007Jul;55(9):976-1000. 3.ShawG.etal(2005)HyperphosphorylatedneurofilamentNF-HisaserumbioMarkerofaxonalinjury.BiochemBiophysResCommun.2005Nov4;336(4):1268-77. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。