Description | DiceristheratelimitingenzymeintheformationofmaturemicroRNAs. |
BatchNo. | Seeproductlabel. |
Unitsize | 100µl |
Antigen | AsyntheticpeptidefrommouseDicerprotein(1388-1405aa)conjugatedtoKLH. |
Sequence | C-NQDKSNSEKWEKDEMTKD |
OtherNames | EndoribonucleaseDicer;Double-strand-specificribonucleasemDCR-1;Dicer1;Mdcr; |
Accession | Q8R418DICER_MOUSE; |
Producedin | Chicken |
Applications | WesternBlotting(WB),Immunohistochemistry(IHC)andImmunoprecipitation(IP).TherecommendedconcentrationforIHCinformalinfixedandparaffinembeddedtissuesandformalin/acetonefixedtissuesis1:200-1:500.ForWB,therecommendedconcentrationis1:1,000to1:3,000.MouseDicerProteinhasapredictedlengthof1916residuesandtheMWofthemonomeris218kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
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Specificity | SpecificityconfirmedbyIHCandWBinmousebrain. |
Cross-reactivity | Mouse;Rat; |
BlastURL | Clickhere |
Form | LyophilisedfromPBS,0.02%sodiumazide |
Reconstitution | Reconstitutein100µlofsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.LugliG.etal(2008)ExpressionofmicroRNAsandtheirprecursorsinsynapticfractionsofadultmouseforebrain.JNeuRochem.Jul;106(2):650-61. 2.LugliG.etal(2005)DicerandeIF2careenrichedatpostsynapticdensitiesinadultmousebrainandaremodifiedbyneuronalactivityinacalpain-dependentmanner.JNeurochem.Aug;94(4):896-905. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。