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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)/C-1373-50/50 µl
产品编号:C-1373-50
市  场 价:¥5940.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$297.00
品      牌: Biosensis
公      司:Biosensis
公司分类:
Biosensis/Chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP)/C-1373-50/50 µl
商品介绍
DescriptionGlialfibrillaryacidicprotein(GFAP)isapprox.50kDaintra-cytoplasmicfilamentousproteinoftheCytoskeletoninastrocytes.Duringthedevelopmentofthecentralnervoussystem,itisacell-specificMarkerthatdistinguishesastrocytesfromotherglialcells.GFAPimmunoreactivityhasbeenshowninimmatureoligodendrocytes,epiglotticcartilage,pituicytes,papillarymeningiomas,myoepithelialcellsofthebreastandinnon-CNS:Schwanncells,salivaryglandneoplasms,entericgliacells,andmetastasizingrenalcarcinomas.
BatchNo.Seeproductlabel
Unitsize50µl
AntigenRecombinantGFAP(expressedinE.coli)andnativebovineGFAP
IsotypeIgY
OtherNamesAstrocyte;Glialfibrillaryacidicprotein;GFAP
AccessionP14136GFAP_HUMAN;Q28115GFAP_BOVIN;
ProducedinChicken
MolecularWeightOnwesternblotsofbrainorspinalcordhomogenateexpecttoseeabandat55kDaandanotheratabout48kDa,apparentlyabreakdownproductoftheprimaryband.
ApplicationsWesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:50,000isrecommendedforWB.HumanGFAPhasapredictedlengthof432residuesandaMWof50kDa.Adilutionof1:1000usingfluorescentsecondaryantibodiesor1:5000usingperoxidaseorotherenzyme-linkedmethodsisrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
ICC:1:1000-1:20,000+,4%PFAfixedcellsinculture,3hr-o/nincubations,optimizationisamustinmostcasesbecauseoftheantibodyhighreactivitytoGFAP
IHC:4%Frozentissues,permeABIlized;IH(P):capable,HEIRtreatmenttypicallynecessary;ChickenIgYcanbindnon-specificallytotissues,specialchickenblocksolutionsavailableelsewherearerecommendedifusedontissues.Dilutions1:5000orgreater;optimizationontime,methodrequiredforbestresults.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWB.
AntibodyAgainstGlialFibrillaryAcidicProtein
Cross-reactivityHuman,Rat,Mouse,Feline.Predictedtoreactwithothermammals.
BlastURLClickhere
FormLyophilisedwith5%trehalose.TheIgYpreparationisataconcentrationof~25mg/mLtotalprotein.
AppearanceWhitepowder
ReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°Cforahigherstability.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
GeneralReferences1.BrennerM.etal(2001)MutationsinGFAP,encodingglialfibrillaryacidicprotein,areassociatedwithAlexanderdisease.NatGenet.2001Jan;27(1):117-20.
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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