Description | TheelF2c(Argonaute,Ago)proteinisacoreproteinoftheRNA-inducedsilencingcomplex(RISC). |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | Asyntheticpeptidecorrespondingtoasequence(845-860aa)fromtheC-terminusofmouseelF2CconjugatedtoKLH. |
Sequence | C-KAVQVHQDTLRTMYFA |
AntigenLocation | 845-860aa(eIF-2C2) |
OtherNames | Proteinargonaute-1;Proteinargonaute-2;Proteinargonaute-3;Argonaute1;Argonaute2;Argonaute3;mAgo1;mAgo2;Eukaryotictranslationinitiationfactor2C;eIF-2C2;eIF-2C1;eIF2C2;Piwi/argonautefamilyproteinmeIF2C2Proteinslicer;Eif2c2;Ago1;Ago2; |
Accession | Q8CJG0AG02_MOUSE; |
Producedin | Rabbit |
Applications | WesternBlotting(WB),Immunochemistry(IHC)andImmunoprecipitation(IP).TherecommendedconcentrationforIHCinformalinfixedandparaffinembeddedtissuesandformalin/acetonefixedtissuesis1:200-1:500.ForWB,therecommendedconcentrationis1:1,000to1:2,000.TheelFC2proteinhasapredictedlengthof860residuesandtheMWofthemonomeris94kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | SpecificityhasbeenconfirmedbyIHC,WBandIPinmousebrain.ThisantibodyisexpectedtorecogniseatleastelF2c-1,2and3sincetheyhaveidenticalC-termini. |
Cross-reactivity | Human;mouse;rat;TheAgoproteinsarehighlyconservedsocross-reactivitywithotherspeciesisexpected. |
BlastURL | Clickhere |
Form | LyophilisedfromPBS |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | HuangKL,ChadeeAB,ChenCY,ZhangY,ShyuAB.(2013)"PhosphorylationatintrinsicallydisorderedregionsofPAM2motif-containingproteinsmodulatestheirinteractionswithPABPC1andinfluencesmRNAfate."RNA.2013Jan22.[Epubaheadofprint]. LugliG.etal(2008)ExpressionofmicroRNAsandtheirprecursorsinsynapticfractionsofadultmouseforebrain.JNeuRochem.Jul;106(2):650-61. LugliG.etal(2005)DicerandeIF2careenrichedatpostsynapticdensitiesinadultmousebrainandaremodifiedbyneuronalactivityinacalpain-dependentmanner.JNeurochem.Aug;94(4):896-905. |
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