Description | Calcium/calmodulin-stimulatedproteinkinaseII(CaMKII)iscomposedoffourdifferentchains(alpha,beta,gamma,anddelta)andisabundantlyexpressedinneurons.CaMKIIisinvolvedinregulatingmanyaspectsofneuronalfunction,includingneurotransmittersynthesisandrelease,modulationofionchannelactivityandcellulartransport.TheenzymaticfunctionofCaMKIIisregulatedbyitsmultiplephosphorylationsitesandtargetingtosub-cellularlocationsthroughinteractionswithproteinbindingpartners.PhosphorylationofThr253hasbeenidentifiedinvivoandfoundtoaltertheinteractionofCaMKIIwithbindingpartners,butnotchangeitsenzymaticactivity.Thus,phosphorylationofThr253issuggestedtomodulatefunctionalresponsesbasedonitsbindingpartnerandsubsequentlyitssub-cellularlocalization. |
BatchNo. | Seeproductlabel |
Unitsize | 100uL |
Antigen | Asyntheticpeptide(NKMLpTINPSC)correspondingtothesequencearoundThr253(AA249-257)inalpha-CaMKIIwassynthesized,purifiedto95%puritybyHPLC,analyzedbymassspectroscopyandcoupledtodiphtheriatoxoid. |
AntibodyType | Polyclonal |
Accession | P11275KCC2A_RAT |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | WesternBlotting(1:2001:1000).Otherapplicationshavenotbeentested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Rat |
Cross-reactivity | Nottested.Predictedfromgeneanalysistoreactwithhumanandmousealpha-CaMKII. |
Form | Lyophilized |
Reconstitution | Reconstitutein100uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.SkeldingKAetal(2012)JJCerebBloodFlowMetab32(12),2181-2192. 2.SkeldingKAetal(2010)CellSignal22(5),759-769. 3.GurdJWetal(2008)BrainRes1218,158-165. 4.MiguesPVetal(2006)JNeuRochem98(1),289-299. |
GeneralReferences | 1.SkeldingKAet(2011)CellCycle10(4),631-639. 2.DosemeciA&JaffeH(2010)BiochemBiophysResCommun391(1),78-84. 3.SkeldingKA&RostasJAP(2009)NeurochemRes34,1792-1804. 4.Dosemeci,A.etal(1994)JBiolChem269(50),31330-31333. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。