BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | AsyntheticpeptidecorrespondingtoasequenceattheN-terminusofhumanHIF-1-alpha(8-21aaNDKKKISSERRKEK),differentfromtherelatedratandmousesequencesbytwoaminoacids. |
OtherNames | HIF-1alpha;HIF1alpha;ARNT-interactingprotein;MemberofPASprotein1;Basic-helix-loop-helix-PASproteinMOP1;HIF1A;MOP1; |
Accession | Q16665HIF1A_HUMAN; |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | WesternBlotting(WB),IHC-P.Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanHIF-1alpha(isoform1)hasapredictedlengthof826residuesandaMWof93kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetectHIF1Ainformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequiredbyboilingtheformalin/paraffinsectionsin10mMcitratebuffer,pH6.0for20mins.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human;rat |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。