Description | Thematrixmetalloproteinases(MMPs)arealargefamilyof zincendopeptidasesthat degradeextracellularmatrixcomponentssuchasfibronectinandcollagentypeIII.Thetissueinhibitorsofmetalloproteinase(TIMPs)complexwiththesematrixmetalloproteinasesandirreversIBLyinactivatethem.TheTIMPproteinsare homologousandareeithersecretedinsolubleform(TIMP1,TIMP2andTIMP4)orbindtoextracellularmatrixcomponents(TIMP3).Metalloproteinaseinhibitor4(TIMP4)isknowntoinhibitMMP-1,MMP-2,MMP-3,MMP-7andMMP-9. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(YRGHLPLRKEFVDIVQP)correspondingtoaregion(208-224)fromtheC-terminusofhumanMetalloproteinaseinhibitor4.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
OtherNames | Tissueinhibitorofmetalloproteinases4;TIMP-4;TIMP4; |
Accession | Q99727TIMP4_HUMAN; |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | WesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanTIMP4(precursor)hasapredictedlengthof224residuesandMWof26kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human;rat;expectedtoreactwithmouseduetosequencehomology; |
Form | Liquid(0.5ml)in50%glycerol,0.9mgNaCland0.2mgNa2HPO4 |
Reconstitution | TheliquidformulationshouldbedilutedinPBS(pH7.4) |
Storage | Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
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