Description | Thematrixmetalloproteinases(MMPs)arealargefamilyof zincendopeptidasesthat degradeextracellularmatrixcomponentssuchasfibronectinandcollagentypeIII.Thetissueinhibitorsofmetalloproteinase(TIMPs)complexwiththesematrixmetalloproteinasesandirreversIBLyinactivatethem.TheTIMPproteinsare homologousandareeithersecretedinsolubleform(TIMP1,TIMP2andTIMP4)orbindtoextracellularmatrixcomponents(TIMP3).Metalloproteinaseinhibitor2(TIMP2)isknowntoinhibitMMP-1,MMP-2,MMP-3,MMP-7,MMP-8,MMP-9,MMP-10,MMP-13,MMP-14,MMP-15,MMP-16andMMP-19. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(RGAAPPKQEFLDIEDP)correspondingtoaregion(205-220)fromtheC-terminusofhumanMetalloproteinaseinhibitor2.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
OtherNames | Tissueinhibitorofmetalloproteinases2;TIMP-2;CSC-21K;TIMP2; |
Accession | P16035TIMP2_HUMAN; |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | WesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanTIMP2(precursor)hasapredictedlengthof220residuesandMWof24kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human;mouse;rat;rabbit; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Liquid(0.5ml).50%glycerol,0.9mgNaCland0.2mgNa2HPO4 |
Reconstitution | TheliquidformulationshouldbedilutedinPBS(pH7.4) |
Storage | Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。