商品介绍
| Description | Humanbeta-endorphinisa31aminoacidpeptidecleavedfromtheprecursorpro-opiomelanocortin(POMC).Itisanendogenousopioidpeptideneurotransmitterthatinteractswithopioidreceptors. |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µg |
| Antigen | Synthetichumanbeta-endorphinpeptide(1-31aa)conjugatedtoBSA. |
| Sequence | YGGFMTSEKSQTPLVTLFKNAIIKNAYKKGE |
| AntigenLocation | 1-31aa |
| OtherNames | -endorphin;Pro-opiomelanocortin;POMC; |
| Accession | P01189COLI_HUMAN; |
| Producedin | Rabbit |
| Applications | Adilutionof5-10µg/mlisrecommendedforimmunohistochemistryusingformalinfixedandparaffinembeddedtissuesandfor4%paraformaldehydefixedfrozentissues.Adilutionof5-15µg/mlisrecommendedforimmunofluorescence.ForrADIoimmunoassay(RIA),aworkingdilutionof1:10,000isrecommended.Sensitivity-120pg/sample;IC50-1ng/sample.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ConfirmedbyRIAagainstthesyntheticpeptide. |
| SpeciesAgainst | Human |
| AntibodyAgainst | betaEndorphin |
| Cross-reactivity | Human;mouse;rat.Beta-endorphinishighlyconservedsocross-reactivitywithotherspeciesisexpected.Cross-reactivitywithotheropioidpeptidesisasfollows:withMet-enkephalin0.03%;withLeu-enkephalin0.02%;withbeta-lipotropin0.34%. |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenproteins.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| BlastURL | Clickhere |
| Form | LyophilisedwithBSA |
| Appearance | Whitepowder |
| Reconstitution | Reconstitutein0.05mlofPBS(pH7.4).Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。
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