Description | Nervegrowthfactor(NGF)issynthesizedasaprecursor(proNGF)whichmaybereleasedandhavephysiologicalfunctionstocausecelldeath.Itbindsneurotrophinreceptorp75andsortilinandmayalsobeimportantforthedevelopmentofnervoussystem.proNGFissynthesizedintargettissuesandglia,transportedretrogradelyandmaybereleased. BiosensisnowoffersbiotinylatedproNGFantibodyallowingmoreflexibilityinexperimentaldesignbyusingthebiotin-avidin/streptavidindetectionmethod.TheABIlityofbiotinylatedproNGFantibodytodetectproNGFhasbeenvalidatedbyWB. |
Relatedproducts | Purified,non-biotinylatedproNGFantibody,cat#M-1738-100 |
BatchNo. | Seeproductlabel |
Unitsize | 50µg |
Antigen | Asyntheticpeptide(C-HTIPQAHWTKLQ,aa:30-41)ofhumanproNGFproteinhasbeenusedastheimmunogen.Thesequenceislocatedonthepro-domainoftheproNGFfull-lengthproteinandis80%homologoustomouseandratproNGF. |
AntibodyType | MousemonoclonalIgGantibody |
Isotype | IgG2b,lamBDa-lightchain |
Clone | BS312 |
OtherNames | Pro-brainnervegrowthfactor;proNGF;NGF |
Accession | NGF_HUMAN |
Producedin | Mouse |
Purity | AntibodywaspurifiedfromcellculturesupernatantbyProteinGchromatography,biotinylatedandbuffer-exchangedintoPBS,pH7.4buffer |
Applications | ThebiotinylatedproNGFantibodyhasbeentestedbyWesternBlotting(0.1-0.5µg/mL)andisalsoexpectedtoworkinapplicationsvalidatedfortheunlabelledantibody(M-1738-100)atsameorhigherdilutions:FlowCytometryandImmunofluorescence. Otherapplicationsnotyettested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Human |
Cross-reactivity | Speciescross-reactivitynottested. |
Conjugate | Biotin |
Form | LyophilisedfromasolutioncontainingPBSbufferpH7.4with3%trehalose,withoutpreservatives. |
Reconstitution | Spinvialbrieflybeforeopening.Reconstitutein50µLofsterile,ultrapurewatertogiveaconcentrationof1mg/mL.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservative. |
Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstability.Antibodycontainsnopreservatives.Storeat4ºCwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | 12monthsafterpurchaseifunopened |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。