Description | Cytokeratinsbelongtotheintermediatefilamentfamilyofproteinsandareclassifiedaccordingtotheirtype.TypeICytokeratinsarelowmolecularweightandacidicwhereasTypeIICytokeratinsarehigherweightandbasictoneutral.ThisantibodyspecificallydetectstypeIICytokeratins1,5,6,and8.ExpressionoftheseCytokeratinsisfrequentlytissuespecific. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Cytokeratinfromhumanepidermis. |
Clone | PC-26 |
OtherNames | Cytokeratin-1;CK-1;KRT1;KRTA;67kDacytokeratin;Hairalphaprotein;Cytokeratin-5;CK-5;Keratin-5;K5;58kDacytokeratin;Cytokeratin-6A;CK6A;K6akeratin;Cytokeratin-6D;CK6D;KRT6A;K6A;KRT6D;Cytokeratin-8;CK-8;Keratin-8;K8;KRT8;CYK8; |
Accession | P04264K2C1_HUMAN;P13647K2C5_HUMAN;P02538K2C6A_HUMAN;P05787K2C8_HUMAN; |
Producedin | Mouse |
Purity | IgG |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanCytokeratin1hasapredictedlengthof644aminoacidsandMWof66kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissuesaswellasformalin/acetonefixedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human;rat; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。