Description | NestinisamemberoftheclassIVintermediatefilamentproteinfamilywhichisexpressedinneuronalstemcells.ThemolecularweightofhumanNestinasdeterminedbySDS-PAGEmobilityisabout240kDa.Howevertherealmolecularweightisconsiderablylessthanthis,at177kDa,thedisparitybeinglikelyduetothehighlychargedregionoftheC-terminalsegment.Nestinisrelativelypoorlyconservedinproteinsequenceacrossspeciesboundaries,sothatthemouseandhumanproteinshaveanoverallidentityofonly62%.Asaresultantibodiestothehumanproteinoftenfailtorecognizetherodenthomologueandviceversa.HoweverthisantibodystainsbothrodentandhumanNestin.AntibodiestoNestinarewidelyusedtoidentifyneuralstemcells. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | Partialsegment(region317-630aa)ofhumanNestinexpressedinE.coli |
AntigenLocation | 317-630 |
AntibodyType | Monoclonal |
Isotype | IgG |
Clone | 4D11 |
OtherNames | Nestin;NES; |
Accession | P48681NEST_HUMAN; |
Producedin | Mouse,IgG1kappa |
Purity | Ascitesfluid |
Applications | WesternBlotting(WB),Immunocytochemistry(IC)andFlowCytometry.SuggesteddilutionforWBis1:1,000-5,000and1:250-500forIC.Use2ug/10^6cellsforFlowCytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Comments | Ready-to-usereagentforin-vitrolaboratoryresearchuseonly. |
Specificity | Thisantibodyisspecificforthe240kDaNestinproteinbyWBondevelopingratbrain(P18)homogenate.Amuchweakerbandatapprox.90kDamayalsobeseen.Thisissuggestedtobeabreakdownproductofthe240kDaband. |
AntibodyAgainst | Nestin |
Cross-reactivity | Human,Rodent |
BlastURL | Clickhere |
Form | Lyophilisedfromascitesfluid. |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | SchomannT,MezzanotteL,DeGrootJCMJ,RivoltaMN,HendriksSH,FrijnsJHM,HuismanMA(2017)Neuronaldifferentiationofhair-follicle-bulge-derivedstemcellsco-culturedwithmousecochlearmodiolusexplants.PLosOne.12(10):e0187183.Application:ICC/IF;Species:Mouse,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs). GhoCG,SchomannT,deGrootSC,FrijnsJH,RivoltaMN,NeumannMH,HuismanMA.(2015)Isolation,expansionandneuraldifferentiationofstemcellsfromhumanpluckedhair-afurthersteptowardsautologousnerverecovery.CytotechnologyInpress.Application:IF;Species:Human,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs),Keywords:Hairfolliclestemcell,Regeneration,Neuralcrest,Neuron,Glia,Cryopreservation |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。