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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Mouse monoclonal antibody to Nestin [4D11] (317-630)/M-1385-100/100 µl
产品编号:M-1385-100
市  场 价:¥5940.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$297.00
品      牌: Biosensis
公      司:Biosensis
公司分类:
Biosensis/Mouse monoclonal antibody to Nestin [4D11] (317-630)/M-1385-100/100 µl
商品介绍
DescriptionNestinisamemberoftheclassIVintermediatefilamentproteinfamilywhichisexpressedinneuronalstemcells.ThemolecularweightofhumanNestinasdeterminedbySDS-PAGEmobilityisabout240kDa.Howevertherealmolecularweightisconsiderablylessthanthis,at177kDa,thedisparitybeinglikelyduetothehighlychargedregionoftheC-terminalsegment.Nestinisrelativelypoorlyconservedinproteinsequenceacrossspeciesboundaries,sothatthemouseandhumanproteinshaveanoverallidentityofonly62%.Asaresultantibodiestothehumanproteinoftenfailtorecognizetherodenthomologueandviceversa.HoweverthisantibodystainsbothrodentandhumanNestin.AntibodiestoNestinarewidelyusedtoidentifyneuralstemcells.
BatchNo.Seeproductlabel
Unitsize100µl
AntigenPartialsegment(region317-630aa)ofhumanNestinexpressedinE.coli
AntigenLocation317-630
AntibodyTypeMonoclonal
IsotypeIgG
Clone4D11
OtherNamesNestin;NES;
AccessionP48681NEST_HUMAN;
ProducedinMouse,IgG1kappa
PurityAscitesfluid
ApplicationsWesternBlotting(WB),Immunocytochemistry(IC)andFlowCytometry.SuggesteddilutionforWBis1:1,000-5,000and1:250-500forIC.Use2ug/10^6cellsforFlowCytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
CommentsReady-to-usereagentforin-vitrolaboratoryresearchuseonly.
SpecificityThisantibodyisspecificforthe240kDaNestinproteinbyWBondevelopingratbrain(P18)homogenate.Amuchweakerbandatapprox.90kDamayalsobeseen.Thisissuggestedtobeabreakdownproductofthe240kDaband.
AntibodyAgainstNestin
Cross-reactivityHuman,Rodent
BlastURLClickhere
FormLyophilisedfromascitesfluid.
AppearanceWhitepowder
ReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
SpecificReferencesSchomannT,MezzanotteL,DeGrootJCMJ,RivoltaMN,HendriksSH,FrijnsJHM,HuismanMA(2017)Neuronaldifferentiationofhair-follicle-bulge-derivedstemcellsco-culturedwithmousecochlearmodiolusexplants.PLosOne.12(10):e0187183.Application:ICC/IF;Species:Mouse,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs).

GhoCG,SchomannT,deGrootSC,FrijnsJH,RivoltaMN,NeumannMH,HuismanMA.(2015)Isolation,expansionandneuraldifferentiationofstemcellsfromhumanpluckedhair-afurthersteptowardsautologousnerverecovery.CytotechnologyInpress.Application:IF;Species:Human,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs),Keywords:Hairfolliclestemcell,Regeneration,Neuralcrest,Neuron,Glia,Cryopreservation
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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