Description | Mucin-1isamembranebound,glycosylatedmucinphosphoprotein.TheMucin-1precursoriscleavedintotwosubunits,alphaandbeta.Thealphasubunithascelladhesiveproperties.Thebetasubunitisinvolvedincellsignaling.Mucin-1isexpressedontheapicalsurfaceofepithelialcellsandinactivatedandunactivatedT-cells.Mucin-1isoverexpressedinepithelialtumorssuchasbreastorovariancancerandalsoinnon-epithelialtumorcells. Atleast10isoformsareproducedbyalternativesplicing. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Humanmilkfatglobulemembranes |
Clone | EMA-39 |
OtherNames | MUC-1;Polymorphicepithelialmucin;PUM;PEM;PEMT;Episialin;Tumor-associatedmucin;Carcinoma-associatedmucin;Tumor-associatedepithelialmembraneantigen;EMA;H23AG;Peanut-reactiveurinarymucin;PUM;Breastcarcinoma-associatedantigenDF3;CD227; |
Accession | P15941MUC1_HUMAN; |
Producedin | Mouse |
Purity | IgG |
Applications | Immunohistochemistry(IHC).Aconcentrationof2.0-4.0µg/mlisrecommendedforIHCtodetecttheproteininformalin/acetonefixedtissuesaswellasformalin/acetonefixedfrozentissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyIHCagainsttheantigen. |
Cross-reactivity | Human; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。