商品介绍
| Description | MAP1AandMAP1Baremicrotubule-associatedproteinwhichmediatethephysicalinteractionsbetweenmicrotubulesandcomponentsoftheCytoskeleton(probablyinvolvedinautophagosomeformation).MAP1AandMAP1Beachconsistofaheavychainsubunitand3differentlightchainsubunits(LC1,LC2andLC3).MAP1LC3A isoneofthelightchainsubunitsandcanassociatewitheitherMAP1AorMAP1B.TheprecursorformofMAP1LC3AiscleavedbyAPG4/ATG4BtoformthecytosolicformLC3-1.ThisisactivatedbyAPG7L/ATG7,transferredtoATG3andconjugatedtophospholipidtoformthemembrane-boundform,LC3-II.MAP1LC3Aismostabundantinheart,brain,liver,skeletalmuscleandtestisbutisabsentinthymusandperipheralleukocytes. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | Asyntheticpeptide(RSFADRCKEVQQI)correspondingtotheN-terminalofhumanMAP1LC3AproteinconjugatedtoBlueCarrierProteinhasbeenusedastheimmunogen.ThesequenceishomologouswithmouseandratMAP1LC3A. |
| OtherNames | Microtubule-associatedproteins1A/1Blightchain3A;MAP1A/MAP1BLC3A;MAP1A/1Blightchain3A;MAP1lightchain3-likeprotein1;Microtubule-associatedprotein1lightchain3alpha;Autophagy-relatedproteinLC3A;Autophagy-relatedubiquitin-likemodifierLC3A;APG8a;MAP1LC3A |
| Accession | MLP3A_HUMAN MLP3A_MOUSE MLP3A_RAT |
| Producedin | Rabbit |
| MolecularWeight | ~20kDauncleaved,14-18kDacleaved,SDS-PAGE |
| Purity | Wholeserum |
| Applications | IHC,immunohistochemistryon4%PFAfixedor2%PLPfixation,1:100-1000primaryantibodydilution.IC,Immunocytochemistry:acetonefixedspecimensrecommended.1:100-1:1000primaryantibodydilution.WesternBlot:R-146-100requiressamplestobedenaturedONLYbyboilinginSDSsolution,notreduced.Nosignalisachievedusingreducedsamples.(SamplestreatedwithDTTorBetamercaptoethanol).1:500overnight4degreesisrecommendedforprimaryantibodydilutioninwesternblots.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | IHC,WBandELISAconfirmedthespecificityforMAP1LC3A. |
| Cross-reactivity | Human,rat.Otherspeciesnotyettested. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20°CforhigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.TheMGCProjectTeam.GenomeRes.14:2121-2127(2004) |
品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。
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