Description | FUNCTION:FunctionsasanE1enzymeessentialformultisubstratessuchasGABARAPL1andATG12.FormsintermediateconjugateswithGABARAPL1(GABARAPL2,GABARAPorMAP1ALC3).FormationofthefinalGABARAPL1-PEconjugateisessentialforautophagy.SUBUNIT:Homodimer(Bysimilarity).InteractswithATG3andATG12.Thecomplex,composedofATG3andATG7,playsaroleintheconjugationofATG12toATG5.SUBCELLULARLOCATION:Cytoplasm(Probable).ALTERNATIVEPRODUCTS:2namedisoformsproducedbyalternativesplicing.TISSUESPECIFICITY:Widelyexpressed,especiallyinkidney,liver,lymphnodesandbonemarrow.DOMAIN:TheC-terminalpartoftheproteinisessentialforthedimerizationandinteractionwithATG3andATG12.SIMILARITY:BelongstotheATG7family.Inyeast,ATG7appearstoberequiredforfusionofperoxisomalandvaculuolarmembranes. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | Asyntheticpeptide(DSTRDRTLDQQC)correspondingtotheC-terminalofhumanAPG7proteinconjugatedtobluecarrierproteinhasbeenusedastheimmunogen.ThepeptideishomologouswiththecorrespondingsequencederivedfromAPG7proteininmouse,rat,S.cerevisiae,Macacamulatta(monkey)andCanisfamiliaris(dog). |
OtherNames | Autophagy-relatedprotein7;Ubiquitin-activatingenzymeE1-likeprotein;hAGP7;APG7-like;ATG7;APG7L |
Accession | ATG7_HUMAN ATG7_MOUSE ATG7_RAT ATG7_Saccharomycescerevisiae ATG7_Macacamulatta ATG7_Canisfamiliaris |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | Non-reducedwesternblot:1:100;IF:acetoneor3.7%PFAfixedcells(HJLimetal2013);1:100;Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | IF,WBandELISAconfirmedthespecificityforATG7. |
Cross-reactivity | Human,rat.Otherspeciesnotyettested. |
Form | Lyophilised |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Storelyophilizedantibodyat2-8ºC.Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | Chiu-WeiChen,etal(2012)Inhibitionofautophagyasatherapeuticstrategyofiron-inducedbraininjuryafterhemorrhageAutophagy,8(10):1510. ChienW.S.etal(2011)SuppressionofautophagyinratliveratlatestageofpolymicrobialsepsisShock.2011Jan14 LeeJ.E.etal(2011)AutophagyRegulatesEmbryonicSurvivalDuringDelayedImplantationEndocrinology.2011Mar1. RyningenAetal(2012)InhibitionofMammaliantargetofrapamycininhumanacutemyeloidleukemiacellshasdiverseeffectsthatdependontheenvironmentalinvitrostress.BoneMarrowRes.2012;2012:329061. OhHAetal(2013)Uncoveringaroleforendocannabinoidsignalinginautophagyinpreimplantationmouseembryos.MolHumReprod.2013Feb;19(2):93-101. |
References | 1.YuanW,etal.Mol.Biol.Cell10:1353-1366(1999). 2.TanidaI,etal.Biochem.Biophys.Res.Commun.292:256-262(2002). 3.TanidaI,etal.J.Biol.Chem.277:13739-13744(2002). 4.MizushimaN,etal.Nature395:395-398(1998). 5.JohnstonM,etal.Science265:2077-2082(1994). 6.HardingT.M,etal.J.CellBiol.131:591-602(1995). 7.KimJ,etal.Mol.Biol.Cell10:1337-1351(1999). 8.KomatsuM,etal.J.Biol.Chem.276:9846-9854(2001). 9.KlionskyD.J,etal.Dev.Cell5:539-545(2003). 10.chimuraY,etal.J.Biol.Chem.279:40584-40592(2004). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。