Description | FUNCTION:Cysteineproteaserequiredforautophagy,whichcleavestheC-terminalpartofeitherMAP1LC3,GABARAPL2orGABARAP,allowingtheliberationofformI.AsubpopulationofformIissubsequentlyconvertedtoasmallerform(formII).FormII,witharevealedC-terminalglycine,isconsideredtobethephosphatidylethanolamine(PE)-conjugatedform,andhasthecapacityforthebindingtoautophagosomes.ENZYMEREGULATION:InhibitedbyN-ethylmaleimide.SUBCELLULARLOCATION:Cytoplasm(Probable). SIMILARITY:BelongstothepeptidaseC54family. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | Asyntheticpeptide(CTVYNSDVIDKQ)correspondingtotheC-terminalofhumanATG4CproteinconjugatedtoBlueCarrierProteinhasbeenusedastheimmunogen.ThepeptideishomologouswiththecorrespondingsequencederivedfromATG4Cproteininmouseandrat. |
OtherNames | CysteineproteaseATG4C;Autophagy-relatedprotein4homologC;Autophagin-3;Autophagy-relatedcysteineendopeptidase3;AUT-like3cysteineendopeptidase;ATG4C;APG4C;AUTL3; |
Accession | ATG4C_HUMAN ATG4C_MOUSE ATG4C_RAT |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | IHC,immunofluorescenceandWB.Adilutionof1:100to1:2000dilutionisrecommendedfortheseapplications.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | IHC,ELISAandWBconfirmedthespecificityforATG4C. |
Cross-reactivity | Human,rat.Otherspeciesnotyettested. |
Form | Lyophilised |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | Sixmonthsafterpurchase |
SpecificReferences | SmuderAJetal.(2011)Exerciseprotectsagainstdoxorubicin-inducedMarkersofautophagysignalinginskeletalmuscle.JApplPhysiol(1985).2011Oct;111(4):1190-8. |
GeneralReferences | 1.MarinoG,etal.J.Biol.Chem.278:3671-3678(2003). 2.OtaT,etal.Nat.Genet.36:40-45(2004). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。