Description | ATG16L1playsanessentialroleinautophagy.ItisahomooligomerantinteractswithATG5.Partofeithertheminorandmajorcomplexesrespectivelycomposedof4setsofATG12-ATG5andATG16L1(400kDa)or8setsofATG12-ATG5andATG16L1(800kDa).APG16L1localisestothecytoplasm,inparticularlytopreautophagosomalstructure(PAS)whereitisinvolvedinthemembranetargetingofATG5.Thereare5namedisoformsproducedbyalternativesplicing.GeneticvariationwithinATG16L1areassociatedwithsusceptibilitytoinflammatoryboweldiseasetype1. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | N-terminalofhumanATG16-L1proteinconjugatedtoBlueCarrierProteinhasbeenusedastheimmunogen. |
OtherNames | Autophagy-relatedprotein16-1;APG16-like1;ATG16L1 |
Accession | A16L1_HUMAN |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | Non-reducedwesternblot:1:100;IP:1-50-1:100(5-10uL/500uLlysate);ELISA:1:2000-1:3000directdetectiononly,recombinantimmunogen. |
Specificity | Detectshumanproteinvianon-reducedWB,andELISA |
Cross-reactivity | Human,rat.Otherspeciesnotyettested. |
Form | Lyophilised |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | Sixmonthsafterpurchase |
SpecificReferences | 1.CooneyRetal(2009).NOD2stimulationinducesautophagyindendriticcellsinfluencingbacterialhandlingandantigenpresentation.Nat.Med.Dec62009 2.ScharlM.etal(2011).Proteintyrosinephosphatasenonreceptortype2regulatesautophagosomeformationinhumanintestinalcells.InflammBowelDis.2011Oct10. |
References | 1.ZhengH,etal.DNASeq.15:303-305(2004). 2.ClarkH.F,etal.GenomeRes.13:2265-2270(2003). |
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