| Description | MAP1AandMAP1Baremicrotubule-associatedproteinwhichmediatethephysicalinteractionsbetweenmicrotubulesandcomponentsoftheCytoskeleton(probablyinvolvedinautophagosomeformation).MAP1AandMAP1Beachconsistofaheavychainsubunitand3differentlightchainsubunits(LC1,LC2andLC3).MAP1LC3AisoneofthelightchainsubunitsandcanassociatewitheitherMAP1AorMAP1B.TheprecursorformofMAP1LC3AiscleavedbyAPG4/ATG4BtoformthecytosolicformLC3-1.ThisisactivatedbyAPG7L/ATG7,transferredtoATG3andconjugatedtophospholipidtoformthemembrane-boundform,LC3-II.MAP1LC3Aismostabundantinheart,brain,liver,skeletalmuscleandtestisbutisabsentinthymusandperipheralleukocytes. |
| BatchNo. | Pleaserefertotheproductlabel. |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(C-RSFADRCKEVQQI)correspondingtoaminoacids11-23ofhumanMAP1LC3Aproteinwasconjugatedtoacarrierproteinandthecomplexusedastheimmunogen.ThishumansequenceishomologouswithmouseandratMAP1LC3A. |
| Sequence | RSFADRCKEVQQI |
| AntigenLocation | Intracellular,cytosolic |
| AntigenLength | 13aminoacids |
| AntibodyType | MousemonoclonalIgG |
| Isotype | IgG2a,k-lightchain |
| Clone | BS405 |
| OtherNames | Microtubule-associatedproteins1A/1Blightchain3A;MAP1A/MAP1BLC3A;MAP1A/1Blightchain3A;MAP1lightchain3-likeprotein1;Microtubule-associatedprotein1lightchain3alpha;Autophagy-relatedproteinLC3A;Autophagy-relatedubiquitin-likemodifierLC3A;APG8a;MAP1LC3A |
| Accession | MLP3A_HUMAN MLP3A_MOUSE MLP3A_RAT |
| Producedin | Mouse |
| MolecularWeight | ThebroadbandseenonWBindicatestheantibodydetectsboththecytosolicLC3-Iandautophagosomemembrane-boundLC3-IIformswithapparentmolecularweightsof~14kDa(LC3-I)and~12kDa(LC3-II). |
| Purity | ProteinGpurifiedmouseimmunoglobulin |
| Applications | FlowCytometry(20µg/mL),Westernblot(10µg/mL),Immunocytochemistry(1-2µg/mL).Otherapplicationshavenotyetbeentested.Biosensisrecommendsoptimaldilutionsandconcentrationsshouldbedeterminedbytheenduser. |
| Specificity | WBconfirmedbindingoftheantibodytoabroadproteinbandwithaMolecularWeightof~14kDa. |
| SpeciesAgainst | Human |
| Cross-reactivity | Rat.TheantibodyisexpectedtoreactwithmouseMAP1LC3Aproteindueto100%sequencehomology. |
| Form | LyophilizedfromPBS,pH7.4,containing3%trehalosewithoutpreservatives. |
| Reconstitution | Reconstitutein100µLofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservatives. |
| Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstABIlity.Antibodycontainsnopreservatives.Storeat4ºCwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。



