Description | MAP1AandMAP1Baremicrotubule-associatedproteinwhichmediatethephysicalinteractionsbetweenmicrotubulesandcomponentsoftheCytoskeleton(probablyinvolvedinautophagosomeformation).MAP1AandMAP1Beachconsistofaheavychainsubunitand3differentlightchainsubunits(LC1,LC2andLC3).MAP1LC3AisoneofthelightchainsubunitsandcanassociatewitheitherMAP1AorMAP1B.TheprecursorformofMAP1LC3AiscleavedbyAPG4/ATG4BtoformthecytosolicformLC3-1.ThisisactivatedbyAPG7L/ATG7,transferredtoATG3andconjugatedtophospholipidtoformthemembrane-boundform,LC3-II.MAP1LC3Aismostabundantinheart,brain,liver,skeletalmuscleandtestisbutisabsentinthymusandperipheralleukocytes. |
BatchNo. | Pleaserefertotheproductlabel. |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(C-RSFADRCKEVQQI)correspondingtoaminoacids11-23ofhumanMAP1LC3Aproteinwasconjugatedtoacarrierproteinandthecomplexusedastheimmunogen.ThishumansequenceishomologouswithmouseandratMAP1LC3A. |
Sequence | RSFADRCKEVQQI |
AntigenLocation | Intracellular,cytosolic |
AntigenLength | 13aminoacids |
AntibodyType | MousemonoclonalIgG |
Isotype | IgG2a,k-lightchain |
Clone | BS405 |
OtherNames | Microtubule-associatedproteins1A/1Blightchain3A;MAP1A/MAP1BLC3A;MAP1A/1Blightchain3A;MAP1lightchain3-likeprotein1;Microtubule-associatedprotein1lightchain3alpha;Autophagy-relatedproteinLC3A;Autophagy-relatedubiquitin-likemodifierLC3A;APG8a;MAP1LC3A |
Accession | MLP3A_HUMAN MLP3A_MOUSE MLP3A_RAT |
Producedin | Mouse |
MolecularWeight | ThebroadbandseenonWBindicatestheantibodydetectsboththecytosolicLC3-Iandautophagosomemembrane-boundLC3-IIformswithapparentmolecularweightsof~14kDa(LC3-I)and~12kDa(LC3-II). |
Purity | ProteinGpurifiedmouseimmunoglobulin |
Applications | FlowCytometry(20µg/mL),Westernblot(10µg/mL),Immunocytochemistry(1-2µg/mL).Otherapplicationshavenotyetbeentested.Biosensisrecommendsoptimaldilutionsandconcentrationsshouldbedeterminedbytheenduser. |
Specificity | WBconfirmedbindingoftheantibodytoabroadproteinbandwithaMolecularWeightof~14kDa. |
SpeciesAgainst | Human |
Cross-reactivity | Rat.TheantibodyisexpectedtoreactwithmouseMAP1LC3Aproteindueto100%sequencehomology. |
Form | LyophilizedfromPBS,pH7.4,containing3%trehalosewithoutpreservatives. |
Reconstitution | Reconstitutein100µLofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservatives. |
Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstABIlity.Antibodycontainsnopreservatives.Storeat4ºCwithanappropriateantibacterialagent.Usesterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | 12monthsafterpurchaseifunopened. |
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