Description | Microtubulesare25nmdiameterproteinrodsfoundinmostkindsofeukaryoticcells.Theyarepolymerizedfromadimericsubunitmadeofone"a"subunitandone"b"tubulinsubunit.Microtubulesareassociatedwithafamilyofproteinscalledmicrotubuleassociatedproteins(MAPs),whichincludestheproteint(tau)andagroupofproteinsreferredtoasMAP1,MAP2,MAP3,MAP4andMAP5.MAP2ismadeupoftwo~280kDaapparentmolecularweightbandsreferredtoasMAP2aandMAP2b.Athirdlowermolecularweightform,usuallycalledMAP2c,correspondstoapairofproteinbandsrunningat~70kDaonSDS-PAGEgels.AlltheseMAP2formsarederivedfromasinglegenebyalternatetranscription,andallshareaC-terminalsequencewhichincludeseitherthreeorfourmicrotubulebindingpeptidesequences,whichareverysimilartothosefoundintherelatedmicrotubulebindingproteint(tau).MAP2isoformsareexpressedonlyinneuronalcellsandspecificallyintheperikaryaanddendritesofthesecells.AntibodiestoMAP2arethereforeexcellentMarkersonneuronalcells,theirperikaryaandneuronaldendrites. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | HighmolecularMAPproteinpreparationderivedfrombovinebrain |
Clone | 5H11 |
OtherNames | Microtubule-associatedprotein2;MAP-2;Mtap2; |
Accession | Q0IIA8MAP2_Bovine |
Producedin | Mouse |
MolecularWeight | http://http://www.uniprot.org/uniprot/Q0IIA8 |
Purity | IgG |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:500-1:1000isrecommendedforIHC.Theoptimaldilutionshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human;Rat;Mouse; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | LyophilizedfromPBS. |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。