Description | Cytochrome c is an important component of the electron transport chain in the mitochondria of all aerobic cells. In addition to its role in oxidative phosphorylation, Cytochrome c is also involved in the initiation of apoptosis. |
Batch No. | See product label |
Unit size | 100 µg |
Antigen | A synthetic peptide (ERADLIAYLKKATNE) corresponding to a region (91-105) from the human cytochrome c. To enhance the immunological response, this peptide was coupled to carrier protein BSA. |
Other Names | CYCS; CYC; |
Accession | P99999 CYC_HUMAN; |
Produced in | Rabbit |
Purity | Affinity purified on antigen column |
Applications | Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/ml is recommended for WB. Human cytochrome c has a predicted length of 105 amino acids and MW of 12 kDa. A concentration of 1.0 µg/ml is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0 µg/ml is recommended to detect the protein in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
Specificity | The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
Cross-reactivity | Human; rat; Predicted to react with mouse due to sequence homology; |
Blast it | If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example. |
Form | Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 |
Reconstitution | Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material. |
Storage | At least 12 months after purchase at 2 - 4°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles |
Expiry Date | 12 months after purchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。