产品编号：R-1342-100
市 场 价：¥6540.00
场 地：美国(厂家直采)
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商品介绍
| Description |  APAF1mediatesthecytochromec-dependentautocatalyticactivationofpro-caspase-9,leADIngtotheactivationofcaspase-3andapoptosis(Ref:SWISSPROT). |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(DAKARNCLLQHREALEKDIKTSYIMDH)correspondingtoaregion(2-28aa)fromtheN-terminusofhumanApoptoticprotease-activatingfactor1(APAF1). |
| Sequence | DAKARNCLLQHREALEKDIKTSYIMDH |
| AntibodyType | Antiserum |
| OtherNames | APAF-1;APAF1;Apoptoticpeptidaseactivatingfactor1; |
| Accession | O14727APAF_HUMAN; |
| Producedin | Rabbit |
| Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0µg/mlisrecommendedforWB.HumanAPAF1hasapredictedlengthof1,248residuesandMWof142kDa.Aconcentrationof2.0µg/mlisrecommendedforIHCtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB(Human)andIHC(Rat)againsttheantigen. |
| AntibodyAgainst | Apoptoticprotease-activatingfactor1 |
| Cross-reactivity | Human(WB);rat(IHC-P);predictedtoreactwithmouseduetosequencehomology; |
| Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenproteins.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
| BlastURL | Clickhere |
| Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
| Appearance | Lyophilizedwhitepowder |
| Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
| Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | SezikMetal.(2016)"Maternalintravenousgranulocyte-colonystimulatingfactorandintra-amniotic1high-doseendotoxinfortheexperimentalcaprinemodeloffetalbraininjury"TurkishJournalofMedicalSciencesDOI:10.3906/sag-1505-28.Application:IHC.Species:Goat AydoganAetal.(2014)"Immunohistochemicalexpressionofcaspase-3,caspase-5,caspase-7andapoptoticprotease-activatingfactor-1(APAF-1)intheliverandkidneyofratsexposedtozoledronicacid(ZOL)andbasicfibroblastgrowthfactor(bFGF)."VetQ.2014;34(3):137-42.Application:IHC.Species:Rat 品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。 公司介绍
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PE-1246-5
TR-100-BG
S-1754-500
Neurotrophic Factors
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PE-1239-5
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2 Plate ELISA kits
M-1763-100-FT
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M-1249-100
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PE-1252-100
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