Description | Mycproto-oncogeneprotein(c-Myc)isatranscriptionfactorwithrolesincellularproliferation,differentiation,apoptosisandcellcycleprogression.Mutationsinthec-Mycgeneandproteinover-expressionhavebeenassociatedwithavarietyofhematopoietictumors,leukemiasandlymphomas. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA)correspondingtoCterminalresidues408-439ofhumanMycproto-oncogeneprotein(c-Myc). |
Clone | IMD-3 |
OtherNames | c-Myc;Transcriptionfactorp64;MYC; |
Accession | P01106MYC_HUMAN; |
Producedin | Mouse |
Purity | IgG |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof4.0µg/mlisrecommendedforWB.Humanc-Myc(isoform1)hasapredictedlengthof439residuesandaMWof49kDa.Aconcentrationof8.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase. |
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