Description | FUNCTION:Involvedinredoxregulationofthecell.Canreducehydrogenperoxideandshortchainorganic,fattyacid,andphospholipidhydroperoxides.Mayplayaroleintheregulationofphospholipidturnoveraswellasinprotectionagainstoxidativeinjury.SUBUNIT:Homotetramer.MayinteractwithHTR2A.SUBCELLULARLOCATION:Cytoplasm.Lysosome.Alsofoundinlungsecretoryorganelles.MISCELLANEOUS:Theactivesiteistheredox-activeCys-47oxidizedtoCys-SOH.Cys-SOHmayrapidlyreactwithaCys-SHoftheothersubunittoformanintermoleculardisulfidewithaconcomitanthomodimerformation.Theenzymemaybesubsequentlyregeneratedbyreductionofthedisulfidebythioredoxin.MISCELLANEOUS:IrreversIBLyinactivatedbyoveroxidationofCys-47(toCys-SO(3)H)uponoxidativestress.SIMILARITY:BelongstotheahpC/TSAfamily.Rehydrinsubfamily. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | RatrecombinantPeroxiredoxin-6 |
OtherNames | Non-seleniumglutathioneperoxidase;antioxidantprotein2;1-Cysperoxiredoxin;1-CysPRX;Acidiccalcium-independentphospholipaseA2;NSGPx;Thiol-specificantioxidantprotein;Prdx6;Aipla2;Aop2;Tsa |
Accession | PRDX6_RAT PRDX6_HUMAN PRDX6_MOUSE |
Producedin | Sheep |
Purity | Wholeserum |
Applications | IHC,WB,ELISA.ThisantibodyworkssuperblyinImmunohistochemistryonfrozenorparaffinembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.Typicalworkingdilutionsforroutineimmunohistochemistryare1:100to1:1000dependingontissueanddetectionmethod.Forwesternblottingadilutionrangeof1:500to1:2000isrecommended.Adilutionof1:1000to1:2000isrecommendedforELISA.Thisantiserumstainsthecytoplasmofepithelialcellsintheratandmouselungandratandhumanbrainastrocytes.ItstainshumanbrainastrocytesinParkinson'sandAlzheimer'sdiseaseandthecentralcoreofsomeLewybodiesinParkinson'sdiseaseanddementiawithLewybodies.Othertissueshavenotyetbeentested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThisantibodyhasbeenshowntobespecificforPeroxiredoxin-6protein. |
Cross-reactivity | Rat,humanandmouse,otherspecieshavenotyetbeentested. |
Blastit | Ifyouwouldliketousethisproductinanotherspeicesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
Form | Lyophilised |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
ExpiryDate | 12monthsafterpurchase |
References | 1.KimT.-S,etal.J.Biol.Chem.272:2542-2550(1997). 2.ChoiH.-J,etal.Nat.Struct.Biol.5:400-406(1998). 3.RushJ,etal.Nat.Biotechnol.23:94-101(2005). 4.WagnerE,etal.Biochem.J.366:777-785(2002). 5.ChenJ.-W,etal.J.Biol.Chem.275:28421-28427(2000). 6.KangS.W,etal.J.Biol.Chem.273:6303-6311(1998). 7.GevaertK,etal.Nat.Biotechnol.21:566-569(2003). 8.GregoryS.G,etal.ature441:315-321(2006). 9.NagaseT,etal.DNARes.2:37-43(1995). 10.FrankS,etal.Oncogene14:915-921(1997). 11.PowerJ,etal.AmJ.Path.161:885-894(2202). 12.PowerJ,etal.ExperimentalLungRes.25:379-391(1999). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。