Description | Glutathioneperoxidase1hasaroleindetoxificationofhydrogenperoxideandisoneofthemostimportantantioxidantenzymesinhumans.Itexistsasahomotetramerwhichlocalisestothecytoplasm.Itbelongstotheglutathioneperoxidasefamily.Glutathioneperoxidase1isoneoffewproteinsinhighervertebratestocontainselenocysteine,whichoccursattheactivesiteofglutathioneperoxidaseandiscodedbyUGA,thatnormallyfunctionsasatranslationterminationcodon.ThisproteinhasapolyalaninesequencepolymorphismintheN-terminalregion,whichincludesthreealleleswithfive,sixorsevenalaninerepeats.Theallelewithfivealaninerepeatsissignificantlyassociatedwithbreastcancerrisk.Atleasttwoalternativelysplicedisoformshavebeenidentified. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | Asyntheticpeptide(RRYSRRFQTIDIEPDIEALL)correspondingtotheaminoacids175-194ofhumanglutathioneperoxidase1(GPx-1)conjugatedtodiphtheriatoxinhasbeenusedastheimmunogen. |
AntibodyType | Polyclonal |
OtherNames | Glutathioneperoxidase1;GPx-1;GSHPx-1;Cellularglutathioneperoxidase;GPX1; |
Accession | P07203GPX1_HUMAN; |
Producedin | Rabbit |
Applications | IHC,WBandELISA.ThisantibodyworkssuperblyinIHConfrozenorwaxembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.TypicalworkingdilutionsforroutineIHCare1:200to1:1,000dependingontissueanddetectionmethod.ForWBadilutionrangeof1:1,000to1:4,000isrecommended.ForELISA,adilutionof1:1,000to1:4,000isrecommended.Thisantiserumhasextremelyhightitre,itstainshumanandratglialcellsintenselyandtosomeextentalsostainsneurons.Othertissueshavenotyetbeentested.OnWBunderreducingconditionsrecognisesa22kDproteininhumanbraintissueandSigmaglutathioneperoxidase(G-4013)isolatedfromhumanerythrocytes.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Humanandratcellularseleniumdependentglutathioneperoxidase. |
Cross-reactivity | Human;rat; |
BlastURL | Clickhere |
Form | Lyophilizedserumwith0.02%thimerosal |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
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