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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/proBDNF Rapid ELISA Kit: Human (2 Plates)/BEK-2237-2P/1 Ea
产品编号:BEK-2237-2P
市  场 价:¥15340.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$767.00
品      牌: Biosensis
公      司:Biosensis
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Biosensis/proBDNF Rapid ELISA Kit: Human (2 Plates)/BEK-2237-2P/1 Ea
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Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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DescriptionTheBiosensisproBDNFRapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthespecific,fastandreliablequantificationofproBDNFinlessthan4hoursincellculturesupernatants,humanserumandEDTA-plasmaonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularhumanbloodsamples.

ThisELISAkitconsistsofapre-coatedmonoclonalanti-proBDNFcaptureantibody,abiotinylatedanti-matureBDNFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3’,5,5’-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofproBDNFpresentinsamplesandproteinstandards.AproBDNFpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance.

ThisproBDNFELISAkitemploysarecombinant,cleavage-resistanthumanproBDNFstandardproducedinmammaliancellsbyBiosensisandvalidatedagainstexternallyavailableproBDNFproteins.

NotethataccurateproBDNFquantificationinhumanserumandEDTA-plasmarequirestheadditionofHeterophilicAntibodyBlockerBL-004-500providedinthekit,andavailableforpurchaseseparately.

ThisELISAkithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedfordiagnosticorclinicalprocedures.
RelatedproductsBL-004-500,Heterophilicantibodyblocker:SamplediluentadditiveforaccuratequantificationofproBDNFinhumanserumandEDTA-plasma.

BEK-2211-2P:MatureBDNFRapid™ELISAKit
BatchNo.Refertotheproductlabel.
AntigenBDNFbelongstotheneurotrophinfamilyandregulatesthesurvivalanddifferentiationofneuronsduringdevelopment.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"sDisease,andParkinson"sdisease.FUNCTION:BDNFpromotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.BDNFisalsoamajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.PostTranslationModification(PTM):ThepropeptideisN-glycosylatedandglycosulfated.PTM:ConvertedintomatureBDNFbyplasmin(PLG).DISEASE:DefectsinBDNFareacauseofcongenitalcentralhypoventilationsyndrome(CCHS);alsoknownascongenitalfailureofautonomiccontrolorOndinecurse.CCHSisararedisordercharacterizedbyabnormalcontrolofrespirationintheabsenceofneuromuscularorlungdisease,oranidentifiablebrainstemlesion.AdeficiencyinautonomiccontrolofrespirationresultsininadequateornegligIBLeventilatoryandarousalresponsestohypercapniaandhypoxemia.CCHSisfrequentlycomplicatedwithneurocristopathiessuchasHirschsprungdiseasethatoccursinabout16%ofCCHScases.SIMILARITY:BelongstotheNGF-betafamily.
OtherNamesBrain-derivedneurotrophicfactor;BDNF;Abrineurin
AccessionP23560BDNF_HUMAN;
SpecificityHumanproBDNF.ThecaptureantibodyusedinthisELISAkitbindstoanepitopewithinthepro-domainofproBDNF.Thus,thisELISAdetectsthefulllengthformofproBDNFanddoesnotquantifymatureBDNF.WhetherthisELISAkitcandetecttruncatedBDNFisunknownatpresent.
Cross-reactivityMatureBDNFcross-reactivitywasassessedbyspiking28kDamatureBDNFproteinobtainedfromWHO(www.NIBSC.org)intoa1/5dilutedhumanserumsampleat5ng/mL,whichrepresentsaBDNFconcentrationlevelof25ng/mLinundiluted,normalhumanserum.Cross-reactivityofmatureBDNFwas< 0.3% (w/v), or < 0.1% in molar concentration.

Theassayantibodiesdonotcross-reactwithnervegrowthfactor(NGF),neurotrophin-3(NT-3)orNT-4/5.

Speciescross-reactivityhasnotyetbeendetermined.
StorageStoreat4°C
ExpiryDate12monthsfrompurchase.
KitcomponentsTheELISAkitboxcontains2x96pre-coatedstripplate,proteinstandards,QCsample,detectionreagents,heterophilicantibodyblocker,washandsamplebuffersanddetailedprotocols.
Range15.6-1000pg/mL
SensitivityTypicallimitofdetection(LOD)forproBDNFis6pg/mLdeterminedas150%oftheblankvalue.
KitprotocolClick to download the kit protocolBEK-2237_as_at_December2017.pdf
MSDSClick to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf