Description | Growth-associatedprotein43(GAP-43)isexpressedbydevelopingandregeneratingneurons,andtoalesserextent,reactiveglialcells. Itisalsoamajorcomponentofneuronalgrowthcones.GAP-43bindscalmodulinwithagreateraffinityintheabsenceofCa(2+)thaninitspresence.PhosphorylationofGAP-43byaproteinKinaseCiscorrelatedwithcertainformsofsynapticplasticity.GAP-43isusedtospecificallylabelinjuredneuronsandtoscoreneuronalregeneration. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptidecorrespondingtoaregion(216-238)fromhumanGrowth-associatedprotein43(GAP-43).Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
OtherNames | Neuromodulin;GAP43;PP46;p57;B-50;F1;AxonalmembraneproteinGAP-43;Growth-associatedprotein43;NeuralphosphoproteinB-50; |
Accession | P17677NEUM_HUMAN |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanGAP-43hasapredictedlengthof238residuesandMWof25kDa.Aconcentrationof0.5-1µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Heatmediatedantigenretrievalisrecommended. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human;rat;predictedtoreactwithmouse; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3addedaspreservative |
Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
ExpiryDate | 12monthsafterpurchase |
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