Description | Fibrillarinispartofthesmallsubunitprocessomecomplex,involvedintheprocessingofpre-18SribosomalRNA.Nop1pistheyeasthomologueoffibrillarin.Fibrillarin/Nop1pisextraordinarilyconserved,sothattheyeastandhumanproteinsare67%identical,andthehumanproteincanfunctionallyreplacetheyeastprotein.Thismeansthatsuitablycross-reactiveantibodiestoNop1p/fibrillarinsuchasthisantibodycanbeusedtorevealnucleoliandstudyfibrillarin/Nop1pinalleukaryotesandarcheatestedtodate.ThisantibodyisbecomingwidelyusedasaconvenientMarkerfornucleoliinawidevarietyofspecies. |
BatchNo. | Seeproductlabel. |
Unitsize | 250µL |
Antigen | Yeastnuclearpreparations.Hybridomaswerescreenedbyimmunofluorescenceonyeastcellsandbywesternblottingonyeastproteinhomogenates(S.cerevisiae). |
AntibodyType | Monoclonal. |
Clone | 38F3 |
OtherNames | rRNA2"-O-methyltransferasefibrillarin;34kDanucleolarsclerodermaantigen;FBL;FIB1;FLRN;Nop1p |
Accession | P15646FBRL_YEAST;P22087FBRL_HUMAN; |
Producedin | Mouse |
Purity | Concentratedcellculturesupernatant. |
Applications | WesternBlotting(WB),Immunocytochemistry(IC).Adilutionof1:2000(celllysates)to1:10000(nuclearfractions)isrecommendedforWBofyeastproteinsamplesfollowedbychemiluminescentdetection(ECL).Forothernon-ECLWBmethods,adilutionof1:1000to1:5000isrecommended.Adilutionof1:500isrecommendedforECLWBonmammalianfibrillarin.Adilutionof1:1000to1:5000isrecommendedforIConyeastcellsand1:500formammaliancells.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB.Thisclonewasselectedbecauseitisspecificforthe~34kDaFibrillarin.Itstainsasinglebandonwesternblottingandshowsaclearandstrongpunctatestainingofyeastnuclei.Itcanthereforebeusedasamarkerfornucleoliinawidevarietyofspecies. |
AntibodyAgainst | Fibrillarin(Nop1p) |
Cross-reactivity | Human,Rat,Drosophila,S.pombe,C.elegansandandS.pombe.Predictedtoreactwithothermammaliantissues. |
Form | Lyophilized,withoutpreservatives. |
Reconstitution | Reconstitutein250uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | PaeschkeK.etal(2005)Telomereend-bindingproteinscontroltheformationofG-quadruplexDNAstructuresinvivo.NatStructMolBiol.2005Oct;12(10):847-54. PostbergJ.etal(2005)Synthesisofpre-rRNAandmRNAisdirectedtoachromatin-poorcompartmentinthemacronucleusofthespirotrichousciliateStylonychialemnae.ChromosomeRes.2006;14(2):161-75. DuT.G.etal(2008)NucleartransitoftheRNA-bindingproteinShe2isrequiredfortranslationalcontroloflocalizedASH1mRNA.EMBORep.2008Aug;9(8):781-7. SrivastavaL.etal(2010)MammalianDEADboxproteinDdx51actsin3"endmaturationof28SrRNAbypromotingthereleaseofU8snoRNA.MolCellBiol.2010Jun;30(12):2947-56. SasanoY.etal(2008)DistributionofU3smallnucleolarRNAandfibrillarinduringearlyembryogenesisinCaenorhaBDitiselegans.Biochimie.2008Jun;90(6):898-907. MengL.etal(2007)Nucleolartraffickingofnucleosteminfamilyproteins:commonversusprotein-specificmechanisms.MolCellBiol.2007Dec;27(24):8670-82. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。